Rice Bran Oil Research Articles

Experimental investigation on nontoxic solvents for separation of vanillic acid

AbstractBACKGROUNDVanillic acid, an aromatic carboxylic acid and a derivative of vanillin, is used as an additive in consumer goods for its aroma. Previously, most vanillic acid was produced through a petrochemical route, but as a consequence of stringent environmental policies, it is now produced by the biotransformation of ferulic acid. Vanillic acid is also found in the wastewater streams of paper‐pulp industries and olive‐oil mills. So, recovering vanillic acid from fermentation broth, either during bioproduction or from a wastewater stream, while minimizing toxicity, is a difficult task. In order to reduce the toxicity substantially, bio‐based solvents such as sunflower, mustard and rice bran oils were employed as diluents along with tri‐n‐butyl phosphate (TBP) as extractant in order to recover vanillic acid by reactive extraction.RESULTSThe extraction performance was elucidated using terms such as distribution ratio, extraction efficiency, loading ratio and equilibrium complexation constant for different extractant–diluent combinations. The highest extraction efficiency and distribution ratio for the different solvents were in the following order: TBP + sunflower‐oil (72.02%–92.47%; 2.57–12.29) > TBP + rice bran‐oil (65.56%–90.55%; 1.90–9.58) > TBP + mustard‐oil(64.03%–89.87%; 1.78–8.87). The theoretical number of stages was determined as 4 to achieve a targeted extraction efficiency of 90%; these predictions were borne out experimentally.CONCLUSIONIn this study, vanillic acid was recovered from the aqueous phase using tri‐n‐butyl phosphate in sustainable, environmentally friendly, bio‐based solvents such as rice bran oil, sunflower oil and mustard oil. © 2024 Society of Chemical Industry (SCI).

Experimental investigation of turning AISI D2 steel using sillimanite (Al2SiO5)

ABSTRACT The global market’s need to decrease costs, minimize health and environmental risks, and achieve cleaner manufacturing has resulted in a growing interest toward hard turning in the manufacturing sector recently. Al2SiO5, Sillimanite is an abundantly available ceramic material with superior properties that is naturally available in the coastal areas of the districts of Odisha and Tamil Nadu, India. In this study, the machining performance while using sillimanite (Al2SiO5) sub-micron particles as MQL additives along with environmentally friendly rice bran oil (RBO) was studied while turning AISI D2. The maximum temperatures at the cutting zones are 371.8°C, 352.4°C & 341°C with SiO2, Al2O3 and Al2SiO5 additives MQL, respectively. With Al2SiO5, the maximum flank wear is 49.9 µm which is significantly less than that of other additives SiO2, Al2O3. Sillimanite, an environmentally friendly ceramic particle with superior mechanical properties when used as an additive for MQL provides greener solution for sustainable machining.

The changes in the physical and chemical quality of catfish oil (Pangasius sp.) with rice bran oil addition during room temperature storage

Catfish oil (CFO) is an oil product that can be obtained through catfish extraction by the dry rendering method and followed by a purification procedure. Fish oil has a high content of unsaturated fatty acid and is susceptible to oxidation and hydrolysis processes leading to a decrease in physical and chemical quality. Rice bran oil (RBO) which is rich in phenolic acid has a good antioxidant potential to inhibit the deterioration of fish oil during storage. This study aimed to determine the effect of the CFO and the RBO ratio on the physical and chemical quality of the CFO with the RBO addition during room temperature storage. This research used a completely randomized design with one factor. Data were analyzed by one-way analysis of variance method and continued by Duncan’s Multiple Range Tests (p<0.05). The ratio of CFO:RBO was 100:0, 90:10, 80:20, 70:30 and 60:40. Data were taken at storage times of weeks 0, 2, 4 and 6. Based on the results obtained, the ratio of 60(CFO):40(RBO) at week 0 has the best physical and chemical quality. It can be concluded that the addition of RBO on CFO effectively inhibits the hydrolysis and oxidation damage up to 6 weeks of room temperature storage.

Electrochemical immunosensor based on antibody-oriented probe for the detection of AFB1 in rice bran oil

Aflatoxin B1 is one of the most toxic mycotoxins, posing a serious threat to human health. There is an urgent need to develop sensitive, rapid and selective ways for the detection of AFB1. Herein, an electrochemical immunosensor was fabricated using the antibody-oriented probe (gold nanoparticle-Streptococcal protein A-anti-AFB1 monoclonal antibody, AuNP-SPA-mAb) to analyze AFB1 in rice bran oil. AuNP-SPA-mAb probe was constructed by the introduction of streptococcal protein A to ensure the controlled immobilization of antibody. AFB1 competed with antigen loaded on the gold electrode for binding AuNP-SPA-mAb in the presence of the target AFB1, inhibiting the capture of horse radish peroxidase (HRP)-labeled second antibody. Subsequently, HRP could facilitate the oxidization of hydroquinone in the presence of hydrogen peroxide, resulting in the production of an electrochemical signal. Under optimal conditions, this immunosensor displayed a linear range of 10–8000 pg/mL and a limit of detection of 2.2 pg/mL. The fabricated immunosensor was further utilized to verify the potential application by detecting samples of rice bran oil. Recovery rates varied from 88.0% to 107.9%. This immunosensor provides a highly sensitive and rapid approach for the determination of such mycotoxins.

Effects of dietary supplementation of grape seed extract in comparison with excessive level of vitamin E on growth performance and antioxidant function of broilers

The present study was carried out to evaluate the effects of dietary vitamin E (VE) or grape seed extract (GSE) on the growth performance and antioxidant function of broilers. Two hundred sixteen broiler chicks were randomly assigned to 3 diets: diet supplemented with oxidized rice bran oil (CN group), CN group with 25 mg/kg VE or 100 mg/kg GSE. Dietary VE or GSE improved the growth performance, reverted the disturbed levels of liver antioxidant enzymes, and reduced liver damage of broilers fed oxidized rice bran oil. The mRNA data showed that supplementation of VE or GSE enhanced the antioxidant capacity of the broiler liver through activation of the Keap1-Nrf2/ARE signaling pathway. The results suggested that VE and GSE can increase weight gain, improve the oxidative status, and alleviate liver injury in broiler chicken fed oxidized rice bran oil.

Formulation and evaluation of bio-grease from the blend of chemically modified rice bran oil and Calophyllum inophyllum oil

Vegetable oils are a highly promising alternative to produce various lubricants, owing to their biodegradability and eco-friendliness. In comparison to mineral oil, these oils possess a higher flash point and viscosity index, along with superior lubricating properties. Additionally, most of the vegetable oils are easily accessible in local markets in India. However, their industrial application is limited by poor thermal and oxidative stability, which can be addressed through chemical modification, the addition of appropriate additives, or by blending these oils. Greases, characterized by their semi-solid consistency, are widely used lubricants. Most of the grease production is based on mineral oils as base oil and lithium soap as a thickener. These materials are not only non-biodegradable but also scarce and have health implications. Consequently, biodegradable grease represents an eco-friendly and healthy alternative. Grease made using vegetable oils with the required properties has the potential to bring about revolutionary changes. The present work focuses on the feasibility of using chemically modified blended rice bran oil (RBO) and Calophyllum inophyllum oil (CIO) as a bio-lubricant. The oils undergo a two-step modification process, involving a transesterification reaction followed by epoxidation. Significant improvements have been observed in the chemical properties (acid, peroxide, and iodine values) of transesterified epoxidized rice bran oil Calophyllum inophyllum oil mixture–50:50 (ETRCIO) when compared with Unmodified Rice bran oil Calophyllum inophyllum oil mixture–50:50 (RCIO). The acid value, peroxide value, and iodine value improved by 91.66%, 87.08%, and 15.78% respectively. The rheological, tribological, and chemical properties of the blended samples have been evaluated and compared to pure oils using American Society for Testing and Materials (ASTM) and Indian Standards (IS). Additionally, ETRCIO was used to develop a bio-grease, and its tribological properties were extensively analyzed. The mean coefficient of friction (COF) and wear scar diameter (WSD) of the ETRCIO grease sample improved by 10.20% and 29.32% respectively when compared with that of commercially available grease. These findings indicate that the ETRCIO bio-grease exhibits superior tribological properties in comparison to commercially available grease.

Quantification of Vitamin A in Edible Oils: Comparison of Portable Device iCheck Chroma3 to High-Performance Liquid Chromatography

AbstractFortification of edible oil with vitamin A is a widely adopted intervention to minimize the effects of vitamin A deficiency in vulnerable groups and mitigate some of its deleterious consequences. Regulatory monitoring is an important prerequisite to ensure that the fortification program is implemented effectively. Standard laboratory analysis methods for vitamin A in oils to assess adequate addition levels remain expensive and time-consuming. Portable testing devices are relatively less expensive in terms of capital investment and cost per test. However, the reliability of results needs to be assured to ensure acceptability and confidence. This study compared a portable device to high-performance liquid chromatography (HPLC) in terms of quantification of vitamin A in both spiked and commercially fortified oils. Nine oils (soybean, palm, cottonseed, rapeseed, corn, peanut, coconut, sunflower, and rice bran oils) were selected and spiked with retinyl palmitate at six different concentrations, and 112 commercially fortified oils were quantified for their vitamin A content using both methods. A good indicator of intra-day and inter-day repeatability (< 10% CV) was obtained for the measurement of vitamin A in the spiked oils for both methods, which denotes a high agreement between them. Vitamin A recoveries were 97–132% for HPLC and 74–127% for the portable device. A strong positive correlation, r = 0.88, is observed between the two methods for the quantification of vitamin A in the commercially fortified oils. The portable device provides a relatively low-cost, quick, and user-friendly alternative to HPLC.

Utilization and Evaluation of Rice Bran and Rice Bran Wax as a Tablet Lubricant.

The rice bran and rice bran wax of the KJ CMU107 rice strain were investigated as potential tablet lubricants in a directly compressed tablet formulation. Stabilized full-fatted rice bran (sFFRB), stabilized defatted rice bran (sDFRB), and rice bran wax (RBW) extracted and purified from crude rice bran oil (cRBO) were tested. Two commercial lubricants, including magnesium stearate (MGS) and hydrogenated cottonseed oil (HVO), were employed as the standards in the formulated mixtures, which contained spray-dried rice starch (SDRS) as a diluent. The tableting was carried out for each formulation, and the obtained tablets were physically and mechanically evaluated. Among the parameters investigated were the general appearance, ejection force, weight variation, hardness, friability, and disintegration time. The powder flow was also determined for each formulation. The results showed that the tablet ejection forces for all the lubricated formulations (58-259 N) were significantly lower than that of the non-lubricated control formulation (349 N). The use of sFFRB as a lubricant at 0.5-2.0% w/w could lower the ejection force up to 78%, but the hardness reduced so drastically that the formulations failed the friability test due to the chipping of the tablets' edges. Moreover, sDFRB performed significantly better as the use at 0.5-1.0% w/w in the formulation helped to lower the ejection forces by up to 80% while maintaining the changes in the tablet hardness within 10%. RBW functioned effectively as a tablet lubricant at a concentration of 0.5% w/w, yielding tablets with good strength comparable to standard HVO lubricant while helping to reduce the ejection force by 82%. In formulations with good lubrication, i.e., friability < 1%, the powder flow was improved, and the tablet disintegration times were within the same range as the control and HVO formulations. In conclusion, sDFRB displayed a lubricant property at concentrations between 0.5 and 1.0% w/w, with slightly negative effects on the tablet hardness. RBW from KJ CMU107 rice was an effective tablet lubricant at 0.5% w/w, with no effect on tablet hardness. Both materials can be further developed for use as commercial lubricants in direct compression.

Carryover of Callosobruchus maculatus (Fab.) in Pigeon Pea Seeds from Field to Storage and its Management by Smearing Oil on Seeds

Background: Pigeonpea, Cajanus cajan (L.) is a vital pulse crop in South Asia, with India accounting for 90% of it’s global production. Its protein content (21-25%) is essential for nutrition and plays significant role in improving food security and nutrition in regions with limited resources. The insect pest attacks in Pigeonpea cause significant amount of grain loss in field and storage, the research was carried out to investigate the carryover of pest from field to storehouses and to study the efficacy of oils on C. maculatus management in which literature available is scarce. Methods: This study was carried out at Sardar Vallabhbhai Patel University of Agriculture and Technology Meerut, U.P., India during the year 2020-2022. Pigeonpea seeds were collected from research farm threshing floor of ICAR-IIFSR, Meerut. Infestations of pulse beetle carried from field to storage was studied on basis of mean no. of eggs laid and mean seed damage caused by beetle. To study efficacy of oils, the seeds were subjected to linseed, mustard, soybean, rice bran, safflower, castor, mahua and neem oil each at concentrations of 0.20, 0.50 and 1.00 ml per 100 g of seeds and five pair of beetles of same age were released into each container and removed after 5 days to study the damage incurred. Result: In the carryover study, maximum oviposition (16.88 eggs) was recorded on freshly harvested seeds that were kept open in storehouse for infestations which witnessed field as well as storage egg laying. Oils application on seeds showed 1ml oil per 100 g seed as most suitable dosage and neem oil, castor, mahua as most successful oil providing beetle control in terms of oviposition, damage and adult survival. Residual action of oil on seed surface after 3 months revealed different oil treatments showed significant reduction in the number of eggs laid, adult emergence and seed damage due to C. maculatus in pigeonpea seeds in comparison to control. Mean reductions in the number of eggs, adult emergence and seed damage were relatively higher in all the oil treatments.

Unleashing the power of hydrous 2-methyl tetrahydrofuran for enhanced oil extraction from rice bran and soybean flakes

Promoting renewable solvents can play a ubiquitous role in sustainable development goals. The investigation delved into the influence of temperature, solvent-to-solid mass ratio, and solvent water content on the yield of rice bran and soybean oil extraction using 2-MeTHF. The JCOSMO software delineated the charge density of solvent and solute molecules. The rice bran oil extracted exhibited a higher free fatty acid content at 26.54 wt%, contrasting with 0.93 wt% for soybean oil. FT-IR analysis was conducted to identify the main functional groups present in the biomasses and oils. The broad and asymmetric XRD peak at 20° decreased in intensity for soybean and remained unchanged for rice bran. Response surface methodology underscored temperature as the most critical variable in oil extraction from both materials, with the solvent-to-solid mass ratio showing no significant impact. Enhanced water content in the solvent proved beneficial for oil extraction from both biomasses. Notably, 2-MeTHF demonstrated higher yields than n-hexane, reaching maximum yields of 20.24 ± 0.64 g/100 g for rice bran and 22.66 ± 0.34 g/100 g for soybean flakes. This study emphasizes critical parameters for achieving higher oil extraction efficiency, highlighting the superior performance of 2-MeTHF over n-hexane. The findings can potentially optimize the extraction process for rice bran and soybean flakes, providing significant benefits for industries reliant on oil. The kinetic investigations substantiated enhanced extraction yields when employing the hydrated solvent; nonetheless, these studies also revealed heightened challenges in attaining equilibrium.

Lipidomics analysis unveils the dynamic alterations of lipid degradation in rice bran during storage

Recent explorations into rice bran oil (RBO) have highlighted its potential, owing to an advantageous fatty acid profile in the context of health and nutrition. Despite this, the susceptibility of rice bran lipids to oxidative degradation during storage remains a critical concern. This study focuses on the evolution of lipid degradation in RBO during storage, examining the increase in free fatty acids (FFAs), the formation of oxylipids, and the generation of volatile secondary oxidation products. Our findings reveal a substantial rise in FFA levels, from 109.55 to 354.06 mg/g, after 14 days of storage, highlighting significant lipid deterioration. Notably, key oxylipids, including 9,10-EpOME, 12,13(9,10)-DiHOME, and 13-oxoODE, were identified, with a demonstrated positive correlation between total oxylipids and free polyunsaturated fatty acids (PUFAs), specifically linoleic acid (LA) and α-linolenic acid (ALA). Furthermore, the study provides a detailed analysis of primary volatile secondary oxidation products. The insights gained from this study not only sheds light on the underlying mechanisms of lipid rancidity in rice bran but also offers significant implications for extending the shelf life and preserving the nutritional quality of RBO, aligning with the increasing global interest in this high-quality oil.

Investigation of the influence of minor components and fatty acid profile of oil on properties of beeswax and stearic acid-based oleogels

Understanding the impact of minor components and the fatty acid profile of oil on oleogel properties is essential for optimizing their characteristics. Considering the scarcity of literature addressing this aspect, this study aimed to explore the correlation between these factors and the properties of beeswax and stearic acid-based oleogels derived from rice bran oil and sesame oil. Minor oil components were modified by stripping the oil, heating the oil with water, and adding β-sitosterol. Oleogels were then prepared using a mixture of beeswax and stearic acid (3:1, w/w) at a concentration of 11.74 % (w/w). The properties of oils and oleogels were evaluated. The findings indicated that minor components and fatty acid composition of the oils substantially influence the oleogel properties. Removing minor components by stripping resulted in smaller and less uniformly distributed crystals and less oil binding capacity compared to the oleogels prepared from untreated oils. A moderate amount of minor components exhibited a significant influence on oleogel properties. The addition of β-sitosterol did not show any influence on oleogel properties except for the oleogel made from untreated oil blend added with β-sitosterol which had more uniform crystals in the microstructure and demonstrated better rheological stability when stored at 5 °C for two months. The oil composition did not show any influence on the thermal and molecular properties of oleogels. Consequently, the oleogel formulation derived from the untreated oil blend enriched with β-sitosterol was identified as the optimal formula for subsequent development. The findings of this study suggest that the physical and mechanical properties as well as the oxidative stability of beeswax and stearic acid-based oleogels are significantly affected by the minor constituents and fatty acid composition of the oil. Moreover, it demonstrates that the properties of oleogels can be tailored by modifying oil composition by blending different oils.

Investigation of Vegetable Oil Blended with Mineral Oil

Oil-based commodities have long dominated the world's energy needs across a variety of industries.. Vegetable-based ester oil is provided as an environmentally friendly substitute for traditional mineral oil for fluid protection. In order to evaluate the essential characteristics of blended liquid as liquid insulation, various ratios of mineral and ester oil were combined in this study. Studies are being conducted on sunflower, safflower, and rice bran oils. Tests are carried out on viscosity, breakdown voltage, flash point, and pour point for different combinations in accordance with industry standards (IEC and ASTM). The study found that blended mixes exhibit quality fluctuations that suggest hopeful signs for mineral oil replacement.

Development of non‐dairy creamer with modified fatty acid profile and high Omega‐3 and 6 at a non‐dairy creamer plant

SummaryA study was conducted to develop non‐dairy creamer production with a modified fatty acid profile having high levels of omega‐3 and 6, by using raw materials collected from the Republic Foods industrial non‐dairy creamer plant. The non‐dairy creamer formulation aimed to maintain a balanced 1:1:1 ratio of polyunsaturated, monounsaturated and saturated fatty acids. The ingredients used included palm olein, stearin, RBD palm oil, rice bran oil, soybean oil, coconut oil and palm kernel oil. The mixture proportions tested were determined using Design Expert software, for in total 20 different formulations. From these, six formulations were selected based on their fatty acid profiles, which closely approximated the desired 1:1:1 ratio of polyunsaturated, monounsaturated and saturated fatty acids. To complement the physicochemical results, a quality control and research officer from Republic Foods industrial conducted a comprehensive evaluation of various properties of the non‐dairy cream samples intended for use in coffee. It is noteworthy that formulation number 20 received the highest scores across all tested properties. This formulation had 50.53% palm olein, 1.00% stearin, 1.00% RBD palm oil, 1.00% rice bran oil, 44.47% soybean oil, 1.00% coconut oil and 1.00% palm kernel oil. This combination results in a fatty acid ratio of 1:1:0.8 (PUFA:MUFA:SFA) at the least production cost. The developed non‐dairy creamer exhibited the highest total energy content, namely 519 kilocalories/100 g, when compared with commercial brands. However, after a storage period of 90 days, the peroxide value reached 6.18 ± 0.22 mEq kg−1 of fat, which is below the FDA standard limit of 10.0 mEq kg−1 fat. This trend in peroxide value increase over the 90‐day storage period aligns with the behaviour of TBA, which also showed an upward trend during storage.

Hypolipidemic Effect of Rice Bran Oil Extract Tocotrienol in High-Fat Diet-Induced Hyperlipidemia Zebrafish (Danio Rerio) Induced by High-Fat Diet.

In recent years, the potent influence of tocotrienol (T3) on diminishing blood glucose and lipid concentrations in both Mus musculus (rats) and Homo sapiens (humans) has been established. However, the comprehensive exploration of tocotrienol's hypolipidemic impact and the corresponding mechanisms in aquatic species remains inadequate. In this study, we established a zebrafish model of a type 2 diabetes mellitus (T2DM) model through high-fat diet administration to zebrafish. In the T2DM zebrafish, the thickness of ocular vascular walls significantly increased compared to the control group, which was mitigated after treatment with T3. Additionally, our findings demonstrate the regulatory effect of T3 on lipid metabolism, leading to the reduced synthesis and storage of adipose tissue in zebrafish. We validated the expression patterns of genes relevant to these processes using RT-qPCR. In the T2DM model, there was an almost two-fold upregulation in pparγ and cyp7a1 mRNA levels, coupled with a significant downregulation in cpt1a mRNA (p < 0.01) compared to the control group. The ELISA revealed that the protein expression levels of Pparγ and Rxrα exhibited a two-fold elevation in the T2DM group relative to the control. In the T3-treated group, Pparγ and Rxrα protein expression levels consistently exhibited a two-fold decrease compared to the model group. Lipid metabolomics showed that T3 could affect the metabolic pathways of zebrafish lipid regulation, including lipid synthesis and decomposition. We provided experimental evidence that T3 could mitigate lipid accumulation in our zebrafish T2DM model. Elucidating the lipid-lowering effects of T3 could help to minimize the detrimental impacts of overfeeding in aquaculture.

Encapsulation of turmeric extract and rice bran oil in alginate hydrogel microcapsules

Encapsulation of turmeric extract and rice bran oil in alginate hydrogel microcapsules

Formulation of Shampoo from Rice Bran Extract (Oryza sativa) and Essential Oil Pomelo Peel (Citrus maxima)

Rice bran (Oryza sativa) is a by-product of rice processing that contains bioactive terpenoid compounds such as oryzanol. This compound has many pharmacological effects such as antioxidant, anti-fungal, anti-bacterial, etc. On the other hand, the essential oil of pomelo peel (Citrus maxima) has a refreshing aroma and also contains a variety of phytochemicals that are efficacious as antioxidants. This study aims to identify optimal formulations of shampoo with a combination of acetone extract from rice bran and essential oil of pomelo peel. Rice bran was extracted using acetone solvent, while essential oil from pomelo peel was obtained by distillation. Phytochemical screening of acetone extract showed the presence of terpenoids, flavonoids, alkaloids, tannins, and saponins. The extracts and essential oil were formulated into shampoo into 4 groups (FI, FII, FIII, FIV), which respectively contained 0%, 10%, 15%, and 20% extract. The results showed the evaluation of the best physical shampoo in Formula III because volunteers preferred Formula III and its better foam stability. Based on these results, it can be concluded that the acetone extract of rice bran and the essential oil of pomelo  peel could be formulated into good shampoo.

Application of Phosphonium- and Choline Chloride Based Deep Eutectic Solvents as Catalyst for Emulsifier Production from Low Quality Oil Assisted by Microwave Irradiation

Monoglycerides (MAG) and Diglycerides (DAG) have wide applications in the food, pharmaceutical and cosmetic industries. Despite the economic competitiveness of producing this type of emulsifier from vegetable oils, the increasing demand for products with high nutritional value makes certain types of healthy oils such as rice bran oil (RBO) a potential raw material for high quality fats to serve this growing market. However, the high free fatty acids (FFA) and RBO content which is insoluble in acetone make it difficult to process and is categorized as a low-quality oil, causing RBO to be only used as biodiesel feedstock. Enzymatic catalytic route for glycerolysis of vegetable oil offers milder operating conditions and higher product selectivity (MAG) but it has high production costs and long processing time. In this study, biodegradable catalysts were used, namely deep eutectic solvents (DES) based on phosponium and choline chloride for glycerolysis of dewaxed/degummed RBO (DDRBO) assisted with microwave irradiation. Effects of different types of DES catalysts (phosphonium and choline chloride based DES) on DES-catalyzed glycerolysis of low quality oil will be evaluated using 5%wt of DES, 300 W(150°C) for about 15 minutes. Out of the four DESs used, DES [TBAB][PTSAM] and DES [ChCl][AcAd] both showed outstanding catalytic performance. Especially [ChCl][AcAd] as a reaction catalyst for DDRBO glycerolysis showed the best catalytic activity, and high selectivity for the formation of monoglycerides (MG). Meanwhile [TBAB][PTSAM] showed high selectivity (92.57%) for diglyceride (DG) formation.

In Vitro Digestibility Assessment of Whey from Goat and Camel Milk Fermented with Lactobacillus helveticus for Use as a Base in Formulating Follow-On Formula.

Follow-on formulas are necessary for newborns that are unable to breastfeed. Thus, the development of formulas more tailored to infants' needs is highly important. Recently, using camel milk, goat milk, and sweet milk whey in the formulation of follow-on formulas has gained researchers' attention. Moreover, developing postbiotic systems to create formulas that mimic human milk, are easy to digest, improve compatibility with an infant's gut, and boost immunity is crucial. Thus, this study aimed to create and assess different formulations using fermented whey from camel and goat milks. The fermentation process involved the use of Lactobacillus helveticus as a probiotic and proteolytic lactic acid bacterium strain. The study monitored the proteolytic activity and antioxidant properties of sweet whey produced from cow, camel, and goat milks during the fermentation process with L. helveticus. Also, three different milk fat blends were recombined using edible vegetable oils (coconut oil, rice bran oil, and canola oil) and then they were used to formulate follow-on formulas with a similar fat composition to human milk. Finally, the prepared formulas were tested for their in vitro digestibility and antioxidant activity before and after digestion. The L. helveticus strain had high proteolytic activity towards whey proteins from all the types of milk used in the study. A fermentation time of 6 h produced a higher proteolytic degree and antioxidant activity than 2 and 4 h of fermentation. No significant differences were observed for proteolytic degree and antioxidant activity between 6 and 12 h of fermentation for the cow, camel, and goat whey samples. Regarding the fat blends, animal milk fat, rice bran oil, and canola oil in a fat combination were essential to provide the required amount of unsaturated fatty acids in the follow-on formulas, especially the linoleic acid-α-linolenic acid (LA:ALA) ratio. Adding coconut oil in small amounts to the follow-on formulas provided the required amounts of saturated fatty acids, especially lauric and meristic acids. The follow-on formula based on cow or goat milk whey fermented with L. helveticus released more free amino acids (mmol tyrosine equivalent mL-1) with high levels of antioxidants compared to unfermented ones. The release of free amino acids in the follow-on formula based on camel milk whey was not affected by fermentation. Our results recommend using L. helveticus in the fermentation of follow-on formulas based on camel and goat whey instead of formulas based on cow milk proteins.

Graphene based T-shaped monopole antenna sensor for food quality evaluation.

Food adulteration has long been considered a major problem. It compromises the quality, safety, and nutritional value of food, posing significant risks to public health. Novel techniques are required to control it. A graphene-based T-shaped monopole antenna sensor was tested for its ability to detect adulteration in liquid foods. Mustard oil was the pure reference sample used for product quality analysis. Olive oil and rice bran oil were adulterants added to the pure sample. It was found that the sensor could be immersed easily in the liquid sample and provided precise results. The graphene-based T-shaped monopole antenna sensor can be used for the quality assessment of liquid food products and is suitable for real-time monitoring. © 2024 Society of Chemical Industry.